Colin Manoil

Professor of Genome Sciences

phone: (206) 543-7800, (206) 543-7795 | fax: (206) 543-0754
Foege S243D, Box 355065
manoil [ a t ]
Photo: Professor Manoil at work on Leave in Bangkok.


Manoil and his colleagues work in the analysis of virulence and antibiotic resistance in three bacterial pathogens (Pseudomonas aeruginosa, Francisella tularensis novicida and Burkholderia pseudomallei). Projects utilize robot-assisted technologies for the generation and phenotype analysis of large sequence-defined transposon mutant libraries.

Selected Publications:

Gallagher, L. A. and C. Manoil (2001). "Pseudomonas aeruginosa PAO1 Kills Caenorhabditis elegans by Cyanide Poisoning." J Bacteriol 183(21): 6207-14.

Bailey, J. and C. Manoil (2002) Genome-wide internal tagging of bacterial exported proteins. Nature Biotechnol. 20: 839-841.

Gallagher, L., McKnight, S, Kuznetsova, M., Pesci, E, and Manoil, C. (2002) Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa. J. Bacteriol., 184: 6472-648.

Jacobs MA, Alwood A, Thaipisuttikul I, Spencer D, Haugen E, Ernst S, Will O, Kaul R, Raymond C, Levy R, Chun-Rong L, Guenthner D, Bovee D, Olson MV, Manoil C (2003) Comprehensive transposon mutant library of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 100:14339-44.

Salama NR, Manoil C. (2006) Seeking completeness in bacterial mutant hunts. Curr Opin Microbiol. 9:307-11.

Gallagher LA, Ramage E , Jacobs MA , Kaul R , Brittnacher M , Manoil C . (2007) A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A. 104:1009-14.

Thongdee, M, Gallagher, L, Schell, M, Dharakul, T, Songsivilai S and Manoil, C (2008) Targeted mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through natural transformation of PCR fragments. App Environ Microbiol, in press.