Research:
Research is conducted in collaboration with Dr. Bonita Brewer. We are studying the regulation of chromosome replication in the yeast Saccharomyces cerevisiae.
The control of DNA replication ensures that chromosomes are duplicated in a timely and precise way. Although the chromosomes of S. cerevisiae (average size, 800 kb) are orders of magnitude smaller, they are organized for replication in much the same way as those of plants and animals: replication occurs from multiple, closely-spaced origins and different parts of a chromosome are replicated at different times during the S phase of the cell cycle. 2-Dimensional gel electrophoresis techniques that we developed allow us to map specific replication origins and to determine the efficiency with which they are activated. Our work is also greatly facilitated by the ability to make directed rearrangements, substitutions and other alterations in yeast plasmids and chromosomes.
Our recent work is concentrated on using hybridization to high density oligonucleotide arrays (''DNA Chips'') to obtain genome-wide information about origin location, time of origin activation, and replication fork progression.
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Publications:
Raghuraman, M.K., Winzeler, E.A., Collingwood, D., Hunt, S., Wodicka, L., Conway, A., Lockhart, D.J., Davis, R.W., Brewer, B.J., Fangman, W.L.. Replication dynamics of the yeast genome. Science. 294: 115-121, 05 Oct 2001
van Brabant A.J., Buchanan C.D., Charboneau E., Fangman W.L., Brewer B.J. An origin-deficient yeast artificial chromosome triggers a cell cycle checkpoint. Molecular Cell. 7(4): 705-13, Apr 2001
Ward T.R., Hoang M.L., Prusty R., Lau C.K., Keil R.L., Fangman W.L., Brewer B.J. Ribosomal DNA replication fork barrier and HOT1 recombination hot spot: shared sequences but independent activities. Molecular and Cellular Biology. 20(13): 4948-57, Jul 2000
van Brabant A.J., Fangman W.L., Brewer B.J. Active role of a human genomic insert in replication of a yeast artificial chromosome.. Mol Cell Biol. 19(6): 4231-40, Jun 1999
Donaldson A.D., Raghuraman M.K., Friedman K.L., Cross F.R., Brewer B. J., Fangman W. L. CLB5-dependent activation of late replication origins in S. cerevisiae.. Mol Cell. 2(2): 173-82, Aug 1998
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