Research Description

Germ cell

Stem-cell-based oocyte (egg) production
The perpetuity of life is carried by germ cells (oocytes and sperm). With the in vitro germ cell development technology, we are approaching the mysteries of germ cells. This progress holds great potential to elucidate the underlying causes of infertility, paving the way for the development of a novel therapy.

Development

Stem-cell-based embryo model 
Embryo development (Embryogenesis) is a sequential process in which single cell results in 37 trillion cells. With in vitro reconstitution of embryo development, we are uncovering the fundamental mechanisms of development. These understandings help us to answer why pregnancy fails in uterus and how we should overcome that threat.

Genomics

High-throughput genomics
We are leveraging the power of advanced genomics technologies to open up new perspectives on biology. In addition to high-throughput experimental genomic technologies, we are developing tools that can record / read / predict / manipulate cellular fate to dissect and understand the complex interplay between the genome and the continuum of normal and abnormal development.

Selected Publications

Hamazaki N, Kyogoku H, Araki H, Miura F, Horikawa C, Hamada N, Shimamoto S, Hikabe O, Nakashima K, Kitajima TS, Ito T, Leitch HG, Hayashi K. Reconstitution of the oocyte transcriptional network with transcription factors. Nature. 2021 Jan;589(7841):264-269. doi: 10.1038/s41586-020-3027-9. Epub 2020 Dec 16. PubMed PMID: 33328630.

Murakami K, Hamazaki N, Hamada N, Nagamatsu G, Okamoto I, Ohta H, Nosaka Y, Ishikura Y, Kitajima TS, Semba Y, Kunisaki Y, Arai F, Akashi K, Saitou M, Kato K, Hayashi K. Generation of functional oocytes from male mice in vitro. Nature. 2023 Mar;615(7954):900-906. doi: 10.1038/s41586-023-05834-x. Epub 2023 Mar 15. PubMed PMID: 36922585.

Hikabe O, Hamazaki N, Nagamatsu G, Obata Y, Hirao Y, Hamada N, Shimamoto S, Imamura T, Nakashima K, Saitou M, Hayashi K. Reconstitution in vitro of the entire cycle of the mouse female germ line. Nature. 2016 Nov 10;539(7628):299-303. doi: 10.1038/nature20104. Epub 2016 Oct 17. PubMed PMID: 27750280.

Qiu C, Martin BK, Welsh IC, Daza RM, Le TM, Huang X, Nichols EK, Taylor ML, Fulton O, Oâ Day DR, Gomes AR, Ilcisin S, Srivatsan S, Deng X, Disteche CM, Noble WS, Hamazaki N, Moens CB, Kimelman D, Cao J, Schier AF, Spielmann M, Murray SA, Trapnell C, Shendure J. A single-cell transcriptional timelapse of mouse embryonic development, from gastrula to pup. bioRxiv. 2023 Apr 5;. doi: 10.1101/2023.04.05.535726. PubMed PMID: 37066300; PubMed Central PMCID: PMC10104014.

Choi J, Chen W, Minkina A, Chardon FM, Suiter CC, Regalado SG, Domcke S, Hamazaki N, Lee C, Martin B, Daza RM, Shendure J. A time-resolved, multi-symbol molecular recorder via sequential genome editing. Nature. 2022 Aug;608(7921):98-107. doi: 10.1038/s41586-022-04922-8. Epub 2022 Jul 6. PubMed PMID: 35794474; PubMed Central PMCID: PMC9352581.

full list:  https://www.ncbi.nlm.nih.gov/myncbi/nobuhiko.hamazaki.1/bibliography/public/