UW Genome Sciences

Research:

Manoil and his colleagues work in the analysis of virulence and antibiotic resistance in three bacterial pathogens (Pseudomonas aeruginosa, Francisella tularensis novicida and Burkholderia pseudomallei). Projects utilize robot-assisted technologies for the generation and phenotype analysis of large sequence-defined transposon mutant libraries.

Selected Publications:

Gallagher, L. A. and C. Manoil (2001). "Pseudomonas aeruginosa PAO1 Kills Caenorhabditis elegans by Cyanide Poisoning." J Bacteriol 183(21): 6207-14.

Bailey, J. and C. Manoil (2002) Genome-wide internal tagging of bacterial exported proteins. Nature Biotechnol. 20: 839-841.

Gallagher, L., McKnight, S, Kuznetsova, M., Pesci, E, and Manoil, C. (2002) Functions Required for Extracellular Quinolone Signaling by Pseudomonas aeruginosa. J. Bacteriol., 184: 6472-648.

Jacobs MA, Alwood A, Thaipisuttikul I, Spencer D, Haugen E, Ernst S, Will O, Kaul R, Raymond C, Levy R, Chun-Rong L, Guenthner D, Bovee D, Olson MV, Manoil C (2003) Comprehensive transposon mutant library of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A. 100:14339-44.

Salama NR, Manoil C. (2006) Seeking completeness in bacterial mutant hunts. Curr Opin Microbiol. 9:307-11.

Gallagher LA, Ramage E , Jacobs MA , Kaul R , Brittnacher M , Manoil C . (2007) A comprehensive transposon mutant library of Francisella novicida, a bioweapon surrogate. Proc Natl Acad Sci U S A. 104:1009-14.

Thongdee, M, Gallagher, L, Schell, M, Dharakul, T, Songsivilai S and Manoil, C (2008) Targeted mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through natural transformation of PCR fragments. App Environ Microbiol, in press.