Publications

Wooden, J., Gould, E. E., Paull, A. T. and Sibley, C H. 1992. Plasmodium falciparum: A Simple Polymerase Chain Reaction Method for Identification of Strains. Experimental Parasitology 75:207-212.

Wooden, J., Kyes, S. and Sibley, C. H. 1993. PCR and Strain Identification in Plasmodium Falciparum. Parasitology Today 9:303-305.

Sibley, C. H. Genetic Analysis of Endotoxin Action. 1993. In: Endotoxin Research Series, Vol. 2, pp. 21-220, J. Lewin, C. R. Alving, R. S. Mienford and P. L. Stutz, eds. Elsevier, Amsterdam.

Rhodes, L. D., Paull, A. T. and Sibley, C. H. 1994. Two Different IFN-g Nonresponsive Variants Derived from the B Cell Lymphoma, 70Z/3. Immunogenetics 40:199--209.

Lawrence, O., Rachie, N., Qureshi, N., Bomsztyk, K. and Sibley, C. H. 1995. Diphosphoryl Lipid A from Rhodobacter sphaeroides Transiently Activates NF-kappa B but Inhibits Lipopolysaccharide (LPS) Induction of Kappa Immunoglobin and Oct-2 in the B Cell Lymphoma 70Z/3. Infection and Immunicty 63:1040-1046.

Wooden, J. M., Hartwell, L. H., Vasquez, B. and Sibley, C. H. 1997. Analysis of Resistance to Inhibitors of Plasmodium falciparum Dihydrofolate Reductase in Yeast. Molec. Biochem. Parasital 85:25-40..

Brophy, V. H. and Sibley, C. H. 1997. Expression of CD14 Corrects the Slow Response to LPS in the B Cell Mutant IB8. Immunogenetics 47, 196-205.

Courtois, G., Whiteside, S.T., Sibley, C.H., and Israel, A. 1997. Characterization of a Mutant Cell Line That Does Not Activate NF-kappa B in Response to Multiple Stimuli. Molecular and Cellular Biology 17:1441-1449.

Sibley, C.H., Brophy, V.H., Cheesman, S., Hamilton, K.L., Hankins, E.G., Wooden, J.M. and Kilbey, B. 1997. Yeast as a Model System to Study Drugs Effective against Apicomplexan Proteins. Methods: A Companion to Methods in Enzymology 13, 190-207.

Nzila-Mounda, A., Mberu, E.K., Sibley, C.H., Plowe, C.V., Winstanley, P.A., Watkins, W.M. 1998. Kenyan Plasmodium falciparum Field Isolates: Correlation between Pyrimethamine and Chlorcycloguanil Activity In Vitro and Point Mutations in the Dihydrofolate Reductase Domain. Antimcrobial Agents and Chemotherapy, 42, 164-169.

Mookherjee, S., Howard, V., Nzila-Mouanda, A., Watkins, W., Sibley, C.H. 1999. Identification and analysis of dihydrofolate reductase alleles from Plasmodium falciparum present at low frequency in polyclonal patient samples. American Journal Tropical Medicine and Hygiene. 61:131-140.

Mberu, E. K., Mosobo, M., Nzila, A., Kokwaro, G. O., Winstanley P. A., Sibley, C.H. and Watkins, W. M. The changing pattern of in-vitro response to pyrimethamine/sulfadoxine in P. falciparum field isolates from Kilifi, Kenya. Journal of American Society of Tropical Medicine and Hygiene.62: 396-401.

Brophy, V.H., Vasquez, J., Nelson, R.G., .Forney, J.R., Rosowsky, A., Sibley, C.H. . 2000. Identification of Cryptosporidium parvum dihydrofolate reductase inhibitors by complementation in Saccharomyces cerevisiae. Antimicrobial Agents and Chemotherapy. 44:1019-1028.

Nzila, A., Mberu, E.K., Sulo, J., Dayo, H. Winstanley, P.W., Sibley C.H., Watkins, W.M. 2000. Towards an understanding of the mechanism of pyrimethamine/sulfadoxine resistance in Plasmodium falciparum: the genotyping of dihydrofolate reductase and dihydropteroate synthase of Kenyan parasites. Antimicrobial Agents and Chemotherapy. 44:991-996.

Nzila, A. M., Nduati, E., Mberu, E.K., Sibley, C.H., Monks, S.A., Winstanley, P.A., and Watkins, W.M. 2000. Molecular evidence of selective pressure for drug resistance exerted by the long acting antifolate, pyrimethamine/sulfadoxine compared with the shorter acting chlorproguanil/dapsone on Kenyan P. falciparum. Journal of Infectious Disease.181:2023-2028.

Macreadie, I.G. and Sibley, C.H. 2001. Yeast enlists in the fight against malaria 2001. Todays Life Sciences. 13:46-50.

Lau, H., Ferlan, J.T., Brophy, V.H., Rosowsky, A. and Sibley, C.H. 2001. Efficacies of lipophilic inhibitors of dihydrofolate reductase against parasitic protozoa. Antimicrobial Agents and Chemotherapy. 45,:187-105.

Ferlan, J.T., Mookherjee, S., Okezie, I.N., Fulgence, l. and Sibley, C.H. 2001. Mutagenesis of dihydrofolate reductase from Plasmodium falciparum: analysis in S. cerevisiae of triple mutant alleles resistant to pyrimethamine or WR99210. Molecular and Biochemical Parasitology. 113:139-150

Sibley, C.H., Hyde, J.E., Sims, P.F.G., Plowe, C.V., Kublin, J.G., Mberu, E.K., Cowman, A.F., Winstanley, P.A., Watkins, W.M., and Nzila, A.M. 2001. Pyrimethamine/sulfadoxine resistance in Plasmodium falciparum: What next? Trends in Parasitology. 17:582-587.

Hankins, E.G., Warhurst, D.C. and Sibley, C.H. 2001. Novel alleles of the P. falciparum dhfr highly resistant to pyrimethamine and chlorcycloguanil, but not WR99210. Molecular and Biochemical Parasitology. 117: 91-102.

Bayly, A. M., J. M. Berglez, O. Patel, L. A. Castelli, E. G. Hankins, P. Coloe, C. H. Sibley, and I. G. Macreadie. 2001. Folic acid utilisation related to sulfa drug resistance in Saccharomyces cerevisiae. FEMS Microbiol Lett 204:387-90.

Quaye, I. And Sibley, C.H. 2002. Molecular Data on Plasmodium falciparum chloroquine and antifolate resistance: a public health tool. Trends in Parasitology 18:184-186

M.D. Hastings, S. J. Bates, E.A. Blackstone, T.K. Mutabingwa and C.H. Sibley. 2002. Highly pyrimethamine-resistant alleles of dihydrofolate reductase in isolates of Plasmodium falciparum from Tanzania. In press , Transactions of the Royal Society of Tropical Medicine and Hygiene

Gerum, A. B., Ulmer, J. E., Jacobus, D. P., Jensen, N. P., Sherman, D. R. and Sibley, C. H. 2002. A novel screen in S. cerevisiae identifies WR99210 analogues that inhibit the dihydrofolate reductase enzyme from Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy 46: 3362-9336

Sibley,C.H. and Hunt, S.Y. Drug resistance in parasites: can we stay ahead of the evolutionary curve? 2003. Trends in Parasitol. 19:532

Rosowsky, A., Forsch, R.A., Sibley, C.H., Inderlied, C.B. and Queener, S. F. 2004. 2,4-Diamino-5-2?, 5?-substituted benzyl)pyrimidines as potential drugs against opportunistic infections. J. Med. Chem. 47, 1475-1486

Hastings, M.D., Porter, K.M., Maguire, J.D. Susanti, I., Kania, W., Bangs, M.J., Sibley, C.H. and Baird, J. K. 2004 Dihydrofolate Reductase Mutations in Plasmodium vivax from Indonesia and Therapeutic Response to Sulfadoxine plus Pyrimethamine. J. Infect. Dis. 189:744-750.

Bates,S.J., Winstanley, P.A., Watkins, W.M., Alloueche, A., Bwika, J., Happi, T.C. Kremsner, P.G., Kublin, J.G., Premji, Z. and Sibley, C.H. 2004. Rare, highly pyrimethamine-resistant alleles of Plasmodium falciparum dihydrofolate reductase from five African sites. Journal of Infectious Diseases. 190: 1783-92.

Watkins,W.M. Sibley,C.H. and Hastings, I.M. 2004. The search for effective and sustainable treatments for falciparum malaria in Africa: A model of the selection of resistance by antifolate drugs and their combinations. In press, American Journal of Tropical Medicine and Hygiene.

Hunt, S.Y., Rezvani, B.B. and Sibley, C.H. 2005. Novel alleles of Plasmodium falciparum dhfr that confer resistance to chlorcycloguanil. Molecular and Biochemical Parasitology. 139:25?32.

Hastings, M.D., Maguire, J.D., Bangs, M.J., Zimmerman, P.A., Reeder, J.C., Baird, J.K., and Sibley, C.H. 2004. Novel Plasmodium vivax dhfr alleles from the Indonesian Archipelago and Papua New Guinea: association with pyrimethamine resistance determined by a yeast expression system. Antimicrobial Agents and Chemotherapy. In press.

Hunt, S.Y., Detering, C., Varani, G. Jacobus, D.P., Schiehser, G.A., Shieh, H.-M., Nevchas, I. Terpinski, J. , Sibley, C.H. Identification of the optimal third generation antifolate against P. falciparum and P. vivax. 2005. Molecular and Biochemical Parasitology. in press.

Sampathkumar, P., Turley, S., Ulmer, J.E., Rhie, H.G., Sibley, C.H., Hol, W.G.H. 2005. Structure of the Mycobacterium tuberculosis flavin dependent thymidylate synthase (MtbThyX) at 2.0 Å resolution. Journal of Molecular Biology. In press

Duffy PE and Sibley CH .2005. Are we losing artemisinin combination therapy already? The Lancet 366:1908-1909.

Hapuarachchi, HC, MY Dayanath, KB Bandara, S Abeysundara, W Abeyewickreme, NR de Silva, SY Hunt, CH Sibley: 2006. Point mutations in the dihydrofolate reductase and dihydropteroate synthase genes of Plasmodium falciparum and resistance to sulfadoxine-pyrimethamine in Sri Lanka . Am J Trop Med Hyg 74:198-204

Sibley, C.H. and Ringwald, P.2006. A database of antimalarial drug resistance: it’s about time. Malaria Journal, 5: 48-.

Sampathkumar, P., Turley, S., Sibley, CH, Hol, WG.J. 2006. NADP + Expels both the Co-factor and a Substrate Analog from the Mycobacterium tuberculosis ThyX Active Site: Opportunities for Anti-bacterial Drug Design. Journal of Molecular Biology. .360:1-6

CH Sibley : Malaria historians: analyze your old slides! 2006. Trends Parasitol, 22: 243-244.

A Attaran, KI Barnes, R Bate, F Binka, U d'Alessandro, CI Fanello, L Garrett, TK Mutabingwa, D Roberts, CH Sibley:2006 The World Bank: false financial and statistical accounts and medical malpractice in malaria treatment. The Lancet, 368: 247-252.

Certain, L and Sibley, CH. 2007. Plasmodium falciparum: A novel method for analyzing haplotypes in mixed infections. Experimental Parasutology, 115:233-241.

Ochong, E, Nzila, AM, Nduati, E, Ochola, I, Kimani, S, Rapuoda, B and Sibley, CH. Longitudinal Analysis of dhfr and dhps genotypes in P. falciparum isolates from six sites in Kenya. submitted to American J of Tropical Medicine and Hygiene.

Certain, LK, Nzila, AM and Sibley, CH. Limited genetic diversity in pyrimethamine resistant strains of Plasmodium falciparum from Kenya . Awaiting KEMRI approval for submission to J Infectious Diseases.

Hawkins,VN Joshi,H, Rungsihirunrat, K., Na-Bangchang, K and Sibley, CH. . Antifolates can have a role in treatment of P. vivax. In press Trends in Parasitology.

Sandefur, CI, Wooden, JM, Quaye, IK, Sirawaraporn, W and Sibley, CH . Pyrimethamine-resistant dihydrofolate reductase enzymes of P. falciparum are not enzymatically compromised in vitro. submitted to Molecular and Biochemical Parasitology.