September 28 Overview of transcription initiation and termination in prokaryotes
Assigned readings:
Young, BA, Gruber, TM and CA Gross (2002) Views of Transcription Initiation. Cell 109:417-420. (Review)
PH von Hippel (1998) An Integrated Model of the Transcription Complex in Elongation, Termination, and Editing. Science 281:660-665. (Review)
Optional background readings:
Alberts, 4 th edition, Molecular Biology of the Cell, Chapter 6
Hartwell et al. 2 nd edition, Genetics, Chapter 8
October 3 Genetic analysis of the lac operon
Assigned readings:
Jacob, F and J Monod (1961) Genetic Regulatory Mechanisms in the Synthesis of Proteins. J Mol Biol 3:318-356.
Preparatory Questions
Assignment
October 5 Transcriptional repression.
Assigned readings:
Gilbert W and B Muller-Hill (1966) Isolation of the Lac Repressor. (1966) PNAS 56:1891-1898.
Lewis et al. (1996) Crystal Structure of the Lactose Operon Repressor and Its Complexes with DNA and Inducer. Science 271:1247-1254.
Optional background readings:
Gilbert W and B Muller-Hill (1967) The Lac Operator is DNA. PNAS 58:2415-2421.
Preparatory Questions
October 12 Transcriptional activation.
Assigned readings:
Niu et al. (1996) Transcription Activation at Class II CAP-Dependent Promoters: Two Interactions between CAP and RNA Polymerase. Cell 87: 1123-1134.
Optional background readings:
Busby S and RH Ebright (1997) Transcription Activation at Class II-Dependent Promoters. Molecular Microbiology 23:853-859.
Lobell RB and RF Schleif (1990) DNA Looping and Unlooping by AraC Protein. Science 250: 528-532.
Eukaryotic Transcription
October 17 Enhancers and transcription initiation in eukaryotes.
Assigned readings:
Carey, M. (1998) The Enhancesome and Transcriptional Synergy. Cell 92: 5-8.
Kim T and T Maniatis (1997) The Mechanism of Transcriptional Synergy of an In Vitro Assembled Interferon-? Enhanceosome. Molecular Cell 1:119-129.
Optional background readings:
Hahn S (2004) Structure and mechanism of the RNA polymerase II transcripion machinery. Nature Structural and Molecular Biology 5:394-403. (Review)
Ptashne M and A Gann (1997) Transcriptional activation by recruitment. Nature 386:569-577. (Review)
Preparatory Questions
Assignment
October 19 Mechanism of the IFB? Enhanceosome
Assigned readings:
Agalioti et al. (2000) Ordered Recruitment of Chromatin Modifying and General Transcription Factors to the IFN-ß Promoter. Cell, 103:667-678.
Munshi et al. (2001) Coordination of a Transcriptional Switch by HMGI (Y) Acetylation. Science, 293: 1133-1136.
Preparatory Questions
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