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M: 9:30-10:50 |
T--QS |
W: 9:30-10:50 |
F: 9:30-10:50 |
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September 29 1. Introduction to Genetics; DNA, the central dogma, protein structure, ORFs, the code, yeast R: 1-12, 161-169, 237-249 Q: 8-9, 13, 16 |
September 30 QS 1. Finding genes in DNA sequence |
October 1 2. Mendelian Segregation; traits, variations in protein function, probability, testcrosses, pedigrees R: 13-24 Q: 2-13, 15, 23, 25, 29 |
October 3 3. Independent Assortment; chi-square analysis R: 25-33, 119 Q: 2-17, 19 |
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October 6 4. Genotype to Phenotype; incomplete dominance, codominance, multiple alleles, pleiotropy, lethality R: 43-53, 213-224 Q: 3-8, 11, 13 |
October 7 QS 2. Cat Genetics Cat of the Day Cat Genetics |
October 8 5. Genetic Interactions; the complementation test, epistsasis, complex traits QS1 Handout due in lecture at 9:30 a.m. R: 53-67, 206-208 Q: 3-15a, 19, 25, 29; 7-21; 18-4 |
October 10 6. Chromosomes; sex ploidy, homologues, mitosis, meiosis R: 77-96, 186, 422-424 Q: 4-5, 10, 12, 13 |
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October 13 7. Chromosome Theory of Inheritance ; sex linkage, aneuploidy R: 98-105, 431-432, 466-468 Q: 4-18, 20, 22, 29, 33 |
October 14 QS 3. Yeast genetics |
October 15 8. Review for Exam I QS2 Handout due in lecture at 9:30 a.m. Key to Nail-Patella Practice Exam Practice Exam Key |
October 17 Exam I: 9:30-11:20 Lectures 1-8 QS 1-3 |
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October 20 9. Linkage vs. Independent Assortment; chromosome mapping, chi-squared analysis R: 113-132 Q: 5-6, 7, 17 (first one), 22, 26, 27 Key to Exam 1a Key to Exam 1b Regrade Policy |
October 21 QS 4. Yeast tetrads SacchDB R: 132-138 |
October 22 10. Molecular Basis for Recombination R: 178-185 Q: 6-23; 7-18 |
October 24 11. DNA Markers; ASO, PCR, restriction enzymes, gels R: 277-285, 297-302, 380-381 Q: 9-25; 10-5; 11-6, 7, 12, 15, 16 |
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October 27 12. Mutation and DNA Repair R: 191-206 Q: 7-3, 7, 13, 31 |
October 28 QS 5. Fly cross -- sex-linked and autosomal traits, mapping R: 813-818 |
October 29 13. Transposons and Chromosomes Rearrangements; P elements, balancer chromosomes, FISH QS4 Handout due in lecture at 9:30 a.m. R: 441-444, 447-466, 817-820 Q: 13-7, 15, 17, 26 |
October 31 14. Mutagenesis in Yeast; the red/ade phenotypes R: Review 213-215; 265-268, 658-659 Q: 7-11, 24, 26; 8-21, 26 |
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November 3 15. Review for Exam II Practice Exam 2 Practice Exam 2 Key |
November 4 QS 6. Identification of mutant flies R: 818-820 |
November 5 EXAM II: 9:30-11:20 Lectures 9-15 QS 4-5 Exam 2a Key Exam 2b Key Regrade Policy |
November 7 16. Recombinant DNA; cloning, genomic and cDNA libraries, clone identification, sequencing R: 285-293, 294-297, 302-307 Q: 9-6, 9, 22, 23, 24 |
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November 10 17. Physical Mapping; restriction mapping, chromosome walking, contigs, Quizzitch computer exercise R: 321-337 Q: 9-12, 14; 10-7, 8, 14 |
November 11 Veteran's Day |
November 12 18. Features of the Human Genome; genes, repeats, deletions, insertions R: 337-348 Q: 10-2, 17 |
November 14 19. DNA polymorphisms and Human Variation; forensics R: 371-387 Q: 11-8, 11, 16 |
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November 17 20. Positional Cloning; mapping human disease genes, LOD scores, northerns R: 387-401, 130-131 Q: 11-2, 18, 24, 25; 18-17 |
November 18 QS 7. Segregation analysis of new fly mutants |
November 19 21. Review for Exam III Practice Exam 3 Practice Exam 3 Key |
November 21 EXAM III: 9:30-11:20 Lectures 16-21 QS 6-7 Quizzitch computer exercise Exam 3a Key Exam 3b Key Regrade Policy |
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November 24 22. Mutant Hunts in Yeast; centromeres and kinetochores R: 415-424 (skim), 424-428, 739-744 Q: 12-13; A-6 |
November 25 QS 8. Genome Center tour UW Genome Center |
November 26 23. Characterizing New Mutants; BLAST, protein homologies, evolution R: 5-8, 714-718 Q: 10-19, 20; 12-10; A-10 |
November 28 Thanksgiving |
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December 1 24. Functional Analysis of Genes; locus-specific mutagenesis R: 744-745, 851-854 Q: Solved problem A-II; E-7 |
December 2 QS 9. Yeast MCM mutants |
December 3 25. Redundant and Non-essential Genes; synthetic lethal screens R: 653-660 Q: A-11 |
December 5 26. Human Disease & Model Organisms; hereditary non-polyposis colon cancer (HNPCC) R: 629-631; Article on hMSH5 Q: 18-18, 20, 21, 22 |
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December 8 27. Human Disease and Model Organisms (cont.) Q: 10-23; 11-10; 12-12; A-12 |
December 9 QS 10. Yeast BLAST search SacchDB NCBI |
December 10 28. Review for Final Exam QS10 Handout due in lecture at 9:30 a.m. Practice Exam 4 Practice Exam 4 Key |
December 12 FINAL EXAM: 8:30-10:20 Lectures 1-28 with emphasis on Lectures 22-28 and QS 8-10 Final Exam Key Exam Pick Up Location |