> pLG122
> 10391 bp
> 
> Features:
> 1 - 1724 (-) mob
> 2188 - 3045 (-) Bla
> 3200 - 4639 (-) transposase
> 4782 - 9840 Transposon T25
>    4782 - 4800 ME
>    4809 - 4856 (+) FRT site
>    5190 - 6026 (+) rhaS
>    5926 - 6948 (+) rhaR
>    7111 - 7700 (-) pMB1 ori
>    7845 - 8639 (-) nptII
>    9024 - 9737 (-) GFP M2
>    9772 - 9819 (+) FRT site
>    9822 - 9840 ME
> 
GATCCAGCCGACCAGGCTTTCCACGCCCGCGTGCCGCTCCATGTCGTTCGCGCGGTTCTCGGAAACGCGCTGCCGCGTTT
CGTGATTGTCACGCTCAAGCCCGTAGTCCCGTTCGAGCGTCGCGCAGAGGTCAGCGAGGGCGCGGTAGGCCCGATACGGC
TCATGGATGGTGTTTCGGGTCGGGTGAATCTTGTTGATGGCGATATGGATGTGCAGGTTGTCGGTGTCGTGATGCACGGC
ACTGACGCGCTGATGCTCGGCGAAGCCAAGCCCAGCGCAGATGCGGTCCTCAATCGCGCGCAACGTCTCCGCGTCGGGCT
TCTCTCCCGCGCGGAAGCTAACCAGCAGGTGATAGGTCTTGTCGGCCTCGGAACGGGTGTTGCCGTGCTGGGTCGCCATC
ACCTCGGCCATGACAGCGGGCAGGGTGTTTGCCTCGCAGTTCGTGACGCGCACGTGACCCAGGCGCTCGGTCTTGCCTTG
CTCGTCGGTGATGTACTTCACCAGCTCCGCGAAGTCGCTCTTCTTGATGGAGCGCATGGGGACGTGCTTGGCAATCACGC
GCACCCCCCGGCCGTTTTAGCGGCTAAAAAAGTCATGGCTCTGCCCTCGGGCGGACCACGCCCATCATGACCTTGCCAAG
CTCGTCCTGCTTCTCTTCGATCTTCGCCAGCAGGGCGAGGATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGG
TGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCC
ACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCCGCCGCCGCCTTTTC
CTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAG
CCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGG
TGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTG
GATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAA
TCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTACCGACTG
GAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGATGCCAAAGAGCTACACCGACGAGCTGGC
CGAGTGGGTTGAATCCCGCGCGGCCAAGAAGCGCCGGCGTGATGAGGCTGCGGTTGCGTTCCTGGCGGTGAGGGCGGATG
TCGAGGCGGCGTTAGCGTCCGGCTATGCGCTCGTCACCATTTGGGAGCACATGCGGGAAACGGGGAAGGTCAAGTTCTCC
TACGAGACGTTCCGCTCGCACGCCAGGCGGCACATCAAGGCCAAGCCCGCCGATGTGCCCGCACCGCAGGCCAAGGCTGC
GGAACCCGCGCCGGCACCCAAGACGCCGGAGCCACGGCGGCCGAAGCAGGGGGGCAAGGCTGAAAAGCCGGCCCCCGCTG
CGGCCCCGACCGGCTTCACCTTCAACCCAACACCGGACAAAAAGGATCCTCTACGCCGGACGCATCGTGGCCGGCATCAC
CGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCA
TGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCTGCCTCGC
GCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCG
GGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGAT
AGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATAAAATCAATCTAAAGTAT
ATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCAT
CCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATA
CCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCC
TGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGC
GCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAA
CGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAG
TAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCT
TTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCA
ACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTC
AAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCA
CCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATA
CTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTAT
TTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGCAGATCTGCAGGTCGACCGATCAG
ATCTTGATCCCCTGCGCCATCAGATCCTTGGCGGCAAGAAAGCCATCCAGTTTACTTTGCAGGGCTTCCCAACCTTCCCA
GAGGGCGCCCCAGCTGGCAATTCCGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACT
GCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGC
ACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAA
GCTTTCTCTGAGCTGTAACAGCCTGACCGCAACAAACGAGAGGATCGAGACCATCCGCTCCAGATTATCCGGCTCCTCCA
TGCGTTGCCTCTCGGCTCCTGCTCCGGTTTTCCATGCCTTATGGAACTCCTCGATCCGCCAGCGATGGGTATAAATGTCG
ATGACGCGCAAGGCTTGGGCTAGCGACTCGACCGGTTCGCTGGTCAGCAACAACCATTTCAACGGGGTCTCACCCTTGGG
CGGGTTAATCTCCTCGGCCAGCACCGCGTTGAGCGTGATATTCCCCTGTTTTAGCGTGATGCGCCCACTGCGCAGGCTCA
AGCTCGCCTTGCGGGCTGGTCGATTTTTACGTTTACCGCGTTTATCCACCACGCCCTTTTGCGGAATGCTGATCTGATAG
CCACCCAACTCCGGTTGGTTCTTCAGATGGTCGTACAGATACAACCCAGACTCTACGTCCTTGCGTGGGTGCTTGGAGCG
CACCACGAAGCGCTCGTTATGCGCCAGTTTGTCCTGCAGATAAGCATGAATATCGGCTTCGCGGTCACAGACCGCAATCA
CGTTGCTCATCATGCTGCCCATGCGTAACCGGCTAGTTGCGGCCGCTGCCAGCCATTTGCCACTCTCCTTTTCATCCGCA
TCGGCAGGGTCATCCGGGCGCATCCACCACTCCTGATGCAGTAATCCTACGGTGCGGAATGTGGTGGCCTCGAGCAAGAG
AACGGAGTGAACCCACCATCCGCGGGATTTATCCTGAATAGAGCCCAGCTTGCCAAGCTCTTCGGCGACCTGGTGGCGAT
AACTCAAAGAGGTGGTGTCCTCAATGGCCAGCAGTTCGGGAAACTCCTGAGCCAACTTGACTGTTTGCATGGCGCCAGCC
TTTCTGATCGCCTCGGCAGAAACGTTGGGATTGCGGATAAATCGGTAAGCGCCTTCCTGGGCGGCTTCACTACCCTCTGA
TGAGATGGGTATTGATTTACCAGAATATTTTGCCAATTGGGCGGCGACGTTAACCAAGCGGGCAGTACGGCGAGGATCAC
CCAGCGCCGCCGAAGAGAACACAGATTTAGCCCAGTCGGCCGCACGATGAAGAGCAGAAGTTATCATGAACGTTACCATG
TTAGGAGGTCACATGGAAGATCAGATCCTGGAAAACGGGAAAGGTTCCGTTCAGGACGCTACTTGTGTACGGGATCGGTC
GACGGATCCCAAGCTTCTTCTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGAATTCCTGTCTCTTATACACATCT
CGCCGACGGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCATTTAAATGGCGCGCCGCTAGGCC
TTACTTGTACAGCTCGTCCACATATGTGATCCTGCTGAATTTCATTACGACCAGTCTAAAAAGCGCCTGAATTCGCGACC
TTCTCGTTACTGACAGGAAAATGGGCCATTGGCAACCAGGGAAAGATGAACGTGATGATGTTCACAATTTGCTGAATTGT
GGTGATGTGATGCTCACCGCATTTCCTGAAAATTCACGCTGTATCTTGAAAAATCGACGTTTTTTACGTGGTTTTCCGTC
GAAAATTTAAGGTAAGAACCTGACCTCGTGATTACTATTTCGCCGTGTTGACGACATCAGGAAGCCAGTATGACCGTATT
ACATAGTGTGGATTTTTTTCCGTCTGGTAACGCGTCCGTGGCGATAGAACCCCGGCTCCCGCAGGCGGATTTTCCTGAAC
ATCATCATGATTTTCATGAAATTGTGATTGTCGAACATGGCACGGGTATTCATGTGTTTAATGGGCAGCCCTATACCATC
ACCGGTGGCACGGTCTGTTTCGTACGCGATCATGATCGGCATCTGTATGAACATACCGATAATCTGTGTCTGACCAATGT
GCTGTATCGCTCGCCGGATCGATTTCAGTTTCTCGCCGGGCTGAATCAGTTGCTGCCACAAGAGCTGGATGGGCAGTATC
CGTCTCACTGGCGCGTTAACCACGGCGTATTGCAGCAGGTGCGACAGCTGGTTGCACAGATGGAACAGCAGGAAGGGGAA
AATGATTTACCCTCGACCGCCAGTCGCGAGATCCTGTTTATGCAATTACTGCTCTTGCTGCGTAAAAGCAGTTTGCAGGA
GAACCTGGAAAACAGCGCATCACGTCTCAACTTGCTTCTGGCCTGGCTGGAGGACCATTTTGCCGATGAGGTGAATTGGG
ATGCCGTGGCGGATCAATTTTCTCTTTCACTGCGTACGCTACATCGGCAGCTTAAGCAGCAAACGGGACTGACGCCTCAG
CGATACCTGAACCGCCTGCGACTGATGAAAGCCCGACATCTGCTACGCCACAGCGAGGCCAGCGTTACTGACATCGCCTA
TCGCTGTGGATTCAGCGACAGTAACCACTTTTCGACGCTTTTTCGCCGAGAGTTTAACTGGTCACCGCGTGATATTCGCC
AGGGACGGGATGGCTTTCTGCAATAACGCGAATCTTCTCAACGTATTTGTACGCCATATTGCGAATAATCAACTTCGTTC
TCTGGCCGAGGTAGCCACGGTGGCGCATCAGTTAAAACTTCTCAAAGATGATTTTTTTGCCAGCGACCAGCAGGCAGTCG
CTGTGGCTGACCGTTATCCGCAAGATGTCTTTGCTGAACATACACATGATTTTTGTGAGCTGGTGATTGTCTGGCGCGGT
AATGGCCTGCATGTACTCAACGATCGCCCTTATCGCATTACCCGTGGCGATCTCTTTTACATTCATGCTGACGATAAACA
CTCCTACGCTTCCGTTAACGATCTGGTTTTGCAGAGTATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGC
AGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGG
CAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCA
GTTGGTGATGTTGCTGAATCGCCATCGTTACACCAGTGATTCGTTGCCGCCAACATCCAGCGAAACGTTGCTGGATAAGC
TGATTACCCGGCTGGCGGCTAGCCTGAAAAGTCCCTTTGCGCTGGATAAATTTTGTGATGAGGCATCGTGCAGTGAGCGC
GTTTTGCGTCAGCAATTTCGCCAGCAGACTGGAATGACCATCAATCAATATCTGCGACAGGTCAGAGTGTGTCATGCGCA
ATATCTTCTCCAGCATAGCCGCCTGTTAATCAGTGATATTTCGACCGAATGTGGCTTTGAAGATAGTAACTATTTTTCGG
TGGTGTTTACCCGGGAAACCGGGATGACGCCCAGCCAGTGGCGTCATCTCAATTCGCAGAAAGATTAAATGCATTTGGTA
ACGAATCAGACAATTGACGGCTTGACGGAGTAGCATAGGGTTTGCAGAATCCCTGCTTCGTCCATTTGACAGGCAGGTAC
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGG
CTCCGCCCCCCTGACGAGCATCACAAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTC
CCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGCCGTTCGCTCCAAGCTGGG
CTGTGTGCACGAACCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACA
CGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAGGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAA
AGAGTTGGTAGCTCTTGATCCGGCAAACAAGCCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCG
CAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAATCGATGAGCTCG
CACGAACCCAGTTGACATAAGCCTGTTCGGTTCGTCCGGACTAGTGATGGAAATGTTGGATCCTCATACTCTTCCTTTTT
CAATTCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCACGAGG
AAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCCGCCAC
ACCCAGCCGGCCACAGTCGATGAATCCAGAAAAGCGGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCATGGG
TCACGACGAGATCCTCGCCGTCGGGCATGCGCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCTGATGCTCT
TCGTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCCGAGTACGTGCTCGCTCGATGCGATGTTTCGCTTGGTGGTC
GAATGGGCAGGTAGCCGGATCAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGGAGCAA
GGTGGGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCGCTTCAGTGACAACGTCGAGC
ACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCTCGTCCTGCAGTTCATTCAGGGCACCGGA
CAGGTCGGTCTTGACAAAAAGAACCGGGCGCCCCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCAGCCGATTGTCT
GTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATCCATCTTGTTCAATCATG
CGAAACGATCCTCATCCTGTCTCTTGATCAGATCTTGATCCCCTGCGCCATCAGATCCTTGGCGGCAAGAAAGCCATCCA
GTTTACTTTGCAGGGCTTCCCAACCTTACCAGAGGGCGCCCCAGCTGGCAATTCCGGTTCGCTTGCTGTCCATAAAACCG
CCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCC
CTTGCGCCATGCATTTGTTAATCGAATTATCCATTGCTGTTGACAAAGGGAATCAGGGGACTATTTGTAGAGCTCATCCA
TGCCATGTGTAATCCCAGCAGCTGTTACAAACTCAAGAAGGACCATGTGGTCTCTCTTTTCGTTGGGATCTTTCGAAAGG
GCAGATTGTGTGGACAGGTAATGGTTGTCTGGTAAAAGGACAGGGCCATCGCCAATTGGAGTATTTTGTTGATAATGGTC
TGCTAGTTGAACGCTTCCATCTTCAATGTTGTGTCTAATTTTGAAGTTAACTTTGATTCCATTCTTTTGTTTGTCTGCCA
TGATGTATACATTGTGTGAGTTATAGTTGTATTCCAATTTGTGTCCAAGAATGTTTCCATCTTCTTTAAAATCAATACCT
TTTAACTCGATTCTATTAACAAGGGCATCACCTTCAAACTTGACTTCAGCACGTGTCTTGTAGTTCCCGTCATCTTTGAA
AAATATAGTTCTTTCCTGTACATAACCTTCGGGCATGGCACTCTTGAAAAAGTCATGCTGTTTCATATGATCTGGGTATC
TCGCAAAGCATTGAAGACCATACGCGAAAGTAGTGACAAGTGTTGGCCATGGAACAGGTAGTTTTCCAGTAGTGCAAATA
AATTTAAGGGTAAGTTTTCCGTATGTTGCATCACCTTCACCCTCTCCACTGACAGAAAATTTGTGCCCATTAACATCACC
ATCTAATTCAACAAGAATTGGGACAACTCCAGTGAAAAGTTCTTCTCCCTTACTCATATGTATATCTCCTTCTTAAATCC
TAGGGCGCGCCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCTTAGATGTGTATAAGAGACAG
CTCGAGAAGGGCGAATTCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGCATGCGATATCGAGCTCTCCCGGGAATTC
CACAAATTGTTATCCGCTCACAATTCCACATGTGGAATTCCACATGTGGAATTCCCATGTCAGCCGTTAAGTGTTCCTGT
GTCACTCAAAATTGCTTTGAGAGGCTCTAAGGGCTTCTCAGTGCGTTACATCCCTGGCTTGTTGTCCACAACCGTTAAAC
CTTAAAAGCTTTAAAAGCCTTATATATTCTTTTTTTTCTTATAAAACTTAAAACCTTAGAGGCTATTTAAGTTGCTGATT
TATATTAATTTTATTGTTCAAACATGAGAGCTTAGTACGTGAAACATGAGAGCTTAGTACGTTAGCCATGAGAGCTTAGT
ACGTTAGCCATGAGGGTTTAGTTCGTTAAACATGAGAGCTTAGTACGTTAAACATGAGAGCTTAGTACGTGAAACATGAG
AGCTTAGTACGTACTATCAACAGGTTGAACTGCTGATCTTCAGATCCTCTACGCCGGACGCATCGTGGCCG