Manoil Lab

F. novicida Mutant Library

The Francisella novicida reduced mutant library exists in two versions:

The two-allele library contains two unique mutant strains for most non-essential genes (Gallagher et al. 2007. PNAS 104(3):1009).  The library and individual strains are available from BEI Resources.

The three-allele library comprises the two-allele set supplemented with additional strains to make a library with an average of three unique mutant strains per non-essential gene (Enstrom M, Held K, Ramage B, Brittnacher M, Gallagher L, Manoil C. Genotype-Phenotype Associations in a Nonmodel Prokaryote. mBio. 2012;3(2):e00001-12. doi:10.1128/mBio.00001-12.).

The accompanying table (F.n. Three Allele Library) lists the members of the three-allele mutant set.  Currently, strains in the three-allele library, which are not part of the two-allele set, may be requested from the Manoil Laboratory.